Abstract

Salvadora persica extract was utilized as a mediate in the synthesis of zinc oxide nanoparticles (ZnO-NPs) under ambient conditions. The biosynthesized particles were characterized via UV-Vis, PXRD, FESEM, EDX, DLS, Raman, and FT-IR analysis. The UV-Vis studies of these particular nanoparticles revealed a characteristic peak at 383 nm, and PXRD pattern presented wurtzite structure (hexagonal) for the synthesized ZnO-NPs, suggesting the formation of ZnO nanoparticles. The FESEM image indicated that the biosynthesized particles were uniformly shaped in hexagonal with the average size of 60-130 nm at calcination temperatures of 500, 600, and 700 degrees C. On the other hand, the EDX clearly exhibited the presence of Zn and O elements in the biosynthesized sample. The powder x-ray diffraction (PXRD) and Raman studies confirmed the formation of single crystalline, hexagonal wurtzite ZnO structures. The cytotoxic activity of the biosynthesized nanoparticles against HT-29 cancer cell line was examined through MTT assay. The results indicated that the toxicity of synthesized nanoparticles is dependent on the concentration of nanoparticles.