Abstract

In recent years, progress of biological synthesis of nanoparticles is inevitable due to its important applications. In this research, a new and simple method for the synthesis of AgNPs from plant extracts is presented. The extract from shoots of the plant Tribulus terrestris L. was mixed with AgNO3 with the aim of biologically synthesizing AgNPs. The biomolecules existing in the extract were accountable for the fast reduction of silver ions (Ag+) to AgNPs. Characterization of biosynthesized AgNPs was performed by UV-Vis, TEM, DLS, and XRD. The AgNPs exhibit a strong peak at 434 nm, and sphere-shaped AgNPs were found to be similar to 25 nm. The biosynthesized silver nanoparticles have demonstrated high antibacterial effect against pathogenic bacteria (i.e., Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa). In addition, the in vitro cytotoxicity effect of biosynthesized silver nanoparticles was also investigated and was detected to be up to 15.62 mu g/mL in the treated Neuro2A cells. The plant-mediated biosynthesis of AgNPs has comparatively rapid, eco-friendly, inexpensive and wide-ranging application in modern medicine and the food industry.