Abstract

In this paper a sensor to detect acetylcholine on the basis of ZnFe2O4 nanoparticles modified screen printed electrode (SPE) is reported. In the range 0.08-500.0 mu M, with the detection limit of 0.024 +/- 0.001 mu M, acquired anodic peak currents where shown to be linearly dependent on acetylcholine concentrations by differential pulse voltammetry (DPV). It was proven that acetylcholine oxidation at adjusted electrode surface takes place at 100 mV potential less positive compared to an unadjusted screen printed electrode. The electron transfer coefficient (alpha=0.51) and diffusion coefficient (D=9.3x10(-6) cm(2)/s) of acetylcholine oxidation were determined too. In addition, this original sensor possesses numerous benefits such as reproducibility, high stability and rapid response (20 s).