Abstract

The efficient ultrasonic route was used to develop novel nanostructures based on Ni/Zn based hybrid MOF. It was characterized using FT-IR, XRD, TEM-EDS, TG and nitrogen adsorption desorption analysis. In comparison to Ni-MOF and Zn-MOF samples, the results showed that Ni/Zn based hybrid MOF nanostructures have a higher surface area and porosity, higher thermal stability and a more uniform morphology. Due to the general importance of enzyme immobilization in industrial applications, Ni/Zn based hybrid MOF nanostructures could be used as an ideal candidate for enzyme immobilization. The effect of experimental parameters on enzyme stabilization was investigated using a 2 k-1 factorial design. The RSM optimization results confirmed the success of immobilization with 93 efficiency. The development of Ni/Zn-based hybrid MOF nanostructures and their remarkable properties such as tunable functionality and well-defined structure into enzyme immobilization could be a new protocol for studying the hosted enzymes.