Repository of Research and Investigative Information

Repository of Research and Investigative Information

Bam University of Medical Sciences

Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu S ricto Complex

(2020) Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus granulosus Sensu S ricto Complex. Iranian Journal of Public Health. pp. 1758-1768. ISSN 2251-6085

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Official URL: <Go to ISI>://WOS:000563786200020

Abstract

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinocuccus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics. Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity. Results: Molecular analysis showed that 50(96.1) and 2 (3.84) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46; G1 genotype) and 2 (3.84; G3 genotype) new haplotypes were unequivocally identified. Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.

Item Type: Article
Keywords: Human hydatidosis Genetic diversity Phylogeography Iran cystic echinococcosis canine echinococcosis infection epidemiology polymorphism genotype Public, Environmental & Occupational Health
Divisions:
Page Range: pp. 1758-1768
Journal or Publication Title: Iranian Journal of Public Health
Journal Index: ISI
Volume: 49
Number: 9
ISSN: 2251-6085
Depositing User: مهندس مهدی شریفی
URI: http://eprints.mubam.ac.ir/id/eprint/1114

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