Abstract

The work descibe a modified screen printed electrode (SPE) for the concurrent analysis of gliclazide and glibenclamide. The modification of the SPE was performed using Fe(3)O(4)nanoparticles (Fe(3)O(4)NPs) derivative. When used for the oxidation of gliclazide under optimal conditions, the over-potential associated with the oxidation of gliclazide dropped for about 130 mV as opposed to the case of unmodified SPEs. The modified electrodes were used to monitor the electrochemical-behavior of gliclazide through differential pulse voltammetry (DPV) and cyclic voltammetry (CV), and also obtain some kinetic data on gliclazide. The response of the electrode was found to be linear from 0.5 to 600.0 mu M and a detection limit of 0.1 mu M (based on 3s/m) was reached through DPV. The Fe3O4 NP/SPE was applied to the determine gliclazide and glibenclamide in the drug and urine samples. The results indicated satisfactory recovery of two analytes. Hence, it was concluded that the electrode is applicable for the analysis of gliclazide and glibenclamide in drug administration and clinical laboratories.